Area (in μm2) occupied by granule cells in the cerebellum proper and ectopically in the midbrain tegmentum and colliculus (extracerebellar) and within the cerebellar white matter (intracerebellar) in mutant control (Unc5h3/Unc5h3;ROSA26), chimera control (+/+↔ROSA26) and experimental chimeric (Unc5h3/Unc5h3↔ROSA26) mice
| Animal | Area of IGL1-a | Area of extracerebellar ectopic granule cells1-b | Area of intracerebellar ectopic granule cells1-c |
|---|---|---|---|
| Unc5h3 mutant; ROSA26 control1-d | 1.10 × 106 | 9.15 × 105 | 6.34 × 105 |
| Control chimeras | |||
| 6 | 2.20 × 106 | None | None |
| 7 | 1.92 × 106 | None | None |
| 8 | 1.98 × 106 | None | None |
| Experimental chimeras | |||
| 1 | 1.75 × 106 | 1.9 × 105 | None |
| 3 | 1.55 × 106 | 6.5 × 105 | 3.26 × 105 |
| 5 | 1.35 × 106 | 4.94 × 105 | 6.00 × 105 |
↵F1-a Mean area of the internal granule cell layer for each animal generated from two medial sections (bins 1 and 2) as described in Materials and Methods.
↵F1-b Mean area of cells in the extracerebellar ectopia for each animal generated from sampled sections in bins 5 and 6 as described in Materials and Methods.
↵F1-c Total area occupied by the granule cells in the intracerebellar ectopias for each animal generated from all 10 of the measured sections as described in Materials and Methods.
↵F1-d Mutant control generated in crosses betweenUnc5h3 and ROSA26 mice as described in Materials and Methods.