Table 1.

HCN mRNA expression in mouse CNS

Brain regionmHCN1mHCN2mHCN4
Olfactory bulb
 Glomerular layer/EPL+/−++++
 Mitral cell layer+/−++++++++
 Granule cell layer/IPL+/−+++/−
Basal forebrain
 Striatum+++
 Globus pallidus+++
 Septum/diagonal band+++++
Amygdala
 Basomedial ant. nucl.+/−++
 Basolateral nucleus+++
 Central nucleus++/−+/−
 Lateral nucleus+/−+/−+
Cerebral cortex
 Neocortex++++++/−
 Piriform cortex+++
Hippocampus
 Pyramidal cells (CA1)++++++/−
 Pyramidal cells (CA3)++++++
 Stratum oriens+++++/−
 Stratum radiatum/L-M+++++/−
 Granule cells (DG)+/−+
 Hilus of dentate gyrus++++
Thalamus
 Anterodorsal nucleus++++++
 Principal relay nuclei+++++++
 Geniculate nuclei++++++
 Nucleus reticularis++++/−
 Lateral habenula++++
 Medial habenula+/−++++
 Subthalamic nucleus+/−++++/−
Hypothalamus
 Preoptic nuclei+++++
 Ventromedial nuclei+++/−+/−
 Mammillary nuclei+++
Midbrain
 Superior colliculus+++++/−
 Inferior colliculus+++++/−
 Substantia nigra+/−+++
Cerebellum
 Molecular cell layer++
 Purkinje cell layer++++
 Granule cell layer++
 Deep cerebellar nuclei+/−++++++
Brainstem
 Vestibular nucleus+/−++++
 Cochlear nucleus++++++
 Spinal trigem. nucl.++++++
 Facial nucleus+++++++
 Hypoglossal nucleus+++++++
 Reticular fields+++++
 Inferior olive++
Spinal cord
 Dorsal horn++++/−
 Ventral horn++++++/−
  • ++++, Very high expression.

  • +++, High expression.

  • ++, Moderate expression.

  • +, Low expression.

  • +/−, Barely above background/background.

  • −, No detectable signal.