Image analysis of DAE-positive (Aβ-positive) structures
| Tg (+) control | Tg (+) ibuprofen | Percent change | p value | ||
|---|---|---|---|---|---|
| Number of plaques | 74.8 ± 13.1 | 35.4 ± 10.5 | ↓52.6% | 0.02 | |
| Mean area of plaques (μm2) | 710.8 ± 96.8 | 671.1 ± 122.1 | No change | 0.85 | |
| Mean % area of plaques | 0.331 ± 0.065 | 0.156 ± 0.05 | ↓52.9% | 0.07 | |
| Total area of plaques (μm2) | 48,485.2 ± 8,523.7 | 21,398.6 ± 6,314.8 | ↓55.9% | 0.02 |
DAE is antibody against Aβ 1-13. Using NIH Image public domain software, data analyzed by two-way ANOVA (diet × region). Analyses were performed on 1570 plaques from Tg+ mice fed control diet (n = 7; three sections per animal) and 461 plaques in Tg+ mice fed ibuprofen diet (n = 5; three sections per animal). Values are mean ± SE. Logarithmic transformation was needed for homogeneity of variance (Bartlett's test) with total area of plaques. Treatment effects were not dependent on region (p = 0.68-98).