Table 1.

Culture of sciatic nerve p75+P0 cells in decreased oxygen promotes neural crest stem cell survival and multilineage differentiation

Plating efficiency (%)Frequency of colony types (%)
N+S+MN+SS+MS onlyM only
20% O235.0  ± 9.148.0  ± 16.41.0  ± 2.14.8  ± 3.443.7  ± 18.52.5  ± 3.0
∼5% O248.0  ± 5.8*82.8  ± 12.0*0.4  ± 1.08.1  ± 7.46.2  ± 3.6*2.5  ± 3.1
  • p75+P0 cells were sorted into culture at clonal density and cultured for 15 d, followed by immunohistochemistry. Plating efficiency indicates the percentage of cells sorted into culture that went on to form colonies detected 15 d later. N, S, and M mean that neurons, Schwann cells, and myofibroblasts, respectively, were observed within colonies. For example, N+S+M colonies contained neurons, Schwann cells, and myofibroblasts. Data are presented as mean ± SD and were derived from six independent experiments in which an average of >70 colonies were counted per treatment per experiment. Results were compared byt tests, and significantly different statistics are noted by asterisks (p < 0.05).