Table 2.

BMP2 instructed neuronal differentiation of sciatic nerve p75+P0 cells is promoted by culture in decreased oxygen

Plating efficiency (%)Types of colonies (%)
All neuronsSome neuronsNo neurons
No add
 20% O222.7  ± 8.50.0  ± 0.00.0  ± 0.0100.0  ± 0.0
 ∼5% O233.4  ± 7.3*0.0  ± 0.00.4  ± 0.999.6  ± 0.9
+BMP2
 20% O226.8  ± 8.931.5  ± 17.952.5  ± 18.915.9  ± 5.7
 ∼5% O243.4  ± 11.5*59.5  ± 10.8*35.0  ± 11.15.5  ± 1.4*
  • p75+P0 cells were sorted into cultures at clonal density and cultured for 5 or 6 d, followed by staining for peripherin, a marker of peripheral neurons. Plating efficiency indicates the percentage of cells initially sorted into culture that went on to form colonies. Data are presented as mean ± SD for five independent experiments. Results at different oxygen concentrations (within no add or +BMP2 treatments) were compared byt tests, and significantly different statistics are noted by asterisks (p < 0.05). Decreased oxygen culture significantly increased plating efficiency in both treatments and neuronal differentiation in the presence of BMP2.