Genotype analysis of progeny from TRAF6 (+/−) intercrosses
| Stage | Number genotyped (percentage) | % TRAF6 (−/−) embryos exhibiting exencephaly | Total number of animals examined at this stage | ||
|---|---|---|---|---|---|
| +/+ | +/− | −/− | |||
| 129J × C57BL6 | |||||
| E12.5 | 4 (18) | 12 (55) | 6 (27) | 17 | 22 |
| E13.5 | 6 (15) | 19 (48) | 15 (37) | 33 | 40 |
| E14.5 | 10 (19) | 27 (50) | 17 (31) | 23 | 54 |
| E15.5 | 15 (27) | 27 (48) | 14 (25) | 29 | 56 |
| E16.5 | 3 (18) | 7 (41) | 7 (41) | 71 | 17 |
| E17.5 | 8 (27) | 15 (50) | 7 (23) | 57 | 30 |
| P14 | 104 (32) | 187 (57) | 36 (11) | 0 | 327 |
| ICR/CD1 | |||||
| P14 | 21 (25) | 46 (55) | 17 (20) | 0 | 84 |
| C57BL6 | |||||
| E18.5 | 2 (20) | 6 (60) | 2 (20) | 100 | 10 |
| P14 | 1-aWild-type allele = 150 | 8 (5) | 0 | 158 | |
TRAF6 (−/−) animals were derived from timed matings of TRAF6 (+/−) mice, with the morning of the plug designated as E0.5. No exencephaly was observed in TRAF6 (+/−) or (+/+) littermates. P14 represents the number of viable progeny observed at P14 derived from TRAF6 (+/−) mice in the mixed C57BL6 × 129J background or backcrossed more than two times onto either C57BL6 or outbred ICR/CD1 genetic backgrounds.
↵F1-a Indicates the number of animals exhibiting one or more wild-type allele(s) from intercrosses of C57BL6 TRAF6 (+/−) mice.