Table 1.

Sleep–wakefulness analysis of animals injected with hcrt1

TreatmentLatency
0–2 h2–4 h
TimenDurationTimenDuration
Wakefulness
 Saline49.8  ± 5.619.1  ± 2.42.9  ± 0.632.9  ± 2.424.5  ± 2.71.5  ± 0.2
 hcrt 1 (25 pmol)85.1  ± 3.5*18.2  ± 2.35.5  ± 0.9*51.3  ± 5.9*34.4  ± 3.4*1.6  ± 0.2
SWS 1
 Saline5.9  ± 0.47.4  ± 0.80.8  ± 0.066.9  ± 1.07.3  ± 0.90.9  ± 0.08
 hcrt 1 (25 pmol)7.9  ± 1.39.2  ± 1.40.8  ± 0.0712.0  ± 2.314.3  ± 2.4*0.8  ± 0.04
SWS 2
 Saline24.5  ± 4.455.9  ± 4.418.1  ± 2.43.3  ± 0.569.9  ± 2.323.4  ± 2.03.1  ± 0.2
 hcrt 1 (25 pmol)52.9  ± 4.6*26.9  ± 3.2*14.4  ± 1.82.0  ± 0.3*55.7  ± 7.030.0  ± 2.92.0  ± 0.4*
REM
 Saline50  ± 6.38.4  ± 1.76  ± 0.81.3  ± 0.210.2  ± 1.16.4  ± 1.01.7  ± 0.1
 hcrt 1 (25 pmol)209.5  ± 24.6*0.05  ± 0.03*0.2  ± 0.1*0.05  ± 0.04*0.9  ± 0.4*1.4  ± 0.6*0.3  ± 0.2*
  • Latency, mean duration, and total time are expressed in minutes ± SEM. Seven animals were analyzed per condition, for 2 hr periods.

  • * Statistical significance of Fisher's exact test (p < 0.05).