Table 3.

Effects of post-training 8-OH-DPAT in 1 and 24 hr tone-dependent memory tests in C57BL/6J mice

Test and test phase Dose (mg/kg)	Test parameters
Test and test phase Dose (mg/kg)	Freezing (%)	IA (%)	mA (cm/sec)	EA (%)
24 hr test
Pre-CS
0	15.98 ± 4.74	47.01 ± 5.16	3.09 ± 0.46	37.85 ± 4.26
0.5	12.51 ± 3.28	36.79 ± 4.39	4.64 ± 0.71	47.34 ± 5.50
1.0	25.00 ± 4.58	44.24 ± 5.65	3.76 ± 0.57	40.11 ± 5.34
CS
0	47.25 ± 6.87	58.03 ± 5.30	2.76 ± 0.40	35.95 ± 5.17
0.5	45.84 ± 6.54	51.38 ± 4.17	3.76 ± 0.65	40.08 ± 4.91
1.0	52.08 ± 6.55	59.21 ± 4.59	2.99 ± 0.43	36.89 ± 5.21
Δ (CS–pre-CS)
0	31.28 ± 5.44	11.01 ± 5.19	−0.33 ± 0.46	−1.90 ± 5.48
0.5	33.33 ± 3.80	14.59 ± 2.39	−0.88 ± 0.23	−7.26 ± 2.53
1.0	27.08 ± 5.79	14.98 ± 4.23	−0.77 ± 0.36	−3.23 ± 4.24
1 hr test
Pre-CS
0	2.78 ± 1.90	19.87 ± 1.72	6.47 ± 0.46	61.72 ± 2.46
0.5	0.00 ± 0.00	17.83 ± 2.06	5.73 ± 0.36	57.65 ± 2.54
1.0	5.57 ± 2.43	30.83 ± 4.90^3-150	4.12 ± 0.47^3-160	42.10 ± 5.88^3-160
CS
0	35.18 ± 5.12	50.00 ± 4.74	3.39 ± 0.45	41.32 ± 5.13
0.5	37.02 ± 4.45	57.55 ± 4.66	2.38 ± 0.35	34.60 ± 3.96
1.0	54.76 ± 6.34^3-150	73.56 ± 4.53^3-160	1.37 ± 0.27^3-165	19.07 ± 5.40^3-160
Δ (CS–pre-CS)
0	32.40 ± 4.41	30.63 ± 5.03	−3.07 ± 0.50	−20.40 ± 4.22
0.5	37.02 ± 4.45	39.72 ± 3.54	−3.34 ± 0.24	−23.05 ± 4.07
1.0	49.19 ± 5.86^3-150	42.73 ± 2.86^3-150	−2.75 ± 0.25	−23.03 ± 4.13

8-OH-DPAT (0.5–1.0 mg/kg, s.c.) was administered directly after conditioning (training). The saline (8 ml/kg, s.c.) control groups were tested concurrently with the respective 8-OH-DPAT-treated groups. Tone-dependent memory tests (pre-CS and CS phases) were performed either immediately after (1 hr test) or 2 hr after (24 hr test) contextual memory tests. The values shown are means (±SEM).
↵F3-150 p < 0.05,
↵F3-160 p < 0.01, and
↵F3-165 p < 0.001 vs corresponding saline control group, n = 6–8. IA, Inactivity; mA, mean activity, EA, exploratory area; Δ (CS–pre-CS), tone-induced change from pre-CS to CS phase. For details, see Materials and Methods and Tables 1 and 2.