Table 2.

Unbiased stereological analysis

Monkey numberDensity of THVolumeTH cell countNon-TH countAll neurons
30 d OVX without estrogen
 16177825.244,79538,54983,345
 17182026.047,38137,22884,609
 18177824.343,16033,78876,948
 Mean179125.245,11236,52281,634
 SD250.92,1282,4584,107
30 d OVX with 30 d estrogen treatment
 19221130.467,11443,622110,736
 20219030.867,52239,210106,732
 21221831.770,35138,138108,490
 Mean220731.068,32940,324108,653
 SD150.71,7632,9062,007
t25914.51.7310.23
p0.00010.00080.0001NS0.0005
 df44444
  • The total number of all neurons, that of TH-immunoreactive cells, their density, and the volume of the compact zone of the substantia nigra unilaterally in different experimental groups are shown.

  • Unbiased stereological analysis of two key groups showed small but significant differences in the total volume of the pars compacta and significant differences between the groups for TH density, total TH cell count, and total number of neurons. There was no significant difference between the groups for the count of non-TH cells.