Table 1.

Inhibition of clathrin assembly and synaptic transmission by AP180 and AP-2 peptides

AP180 peptidesSequenceIC50 for AP-2- mediated assemblyIC50 for AP180- mediated assemblyMean inhibition of transmission
AP180 pep SGGATAWGDLLGEDSLAALSS0.4 mm0.5 mm57%  ± 5.8 (20)1-a
AP180 pep ΔDLL SGGATAWG AAAGEDSLAALSS3.11-b4.91-b0 (2)
Scram AP180 pep 1 SG LLDSADAEGLTAAWGLGSSND1-c1.81-b6.6  ± 2.8 (5)
Scram AP180 pep 2 GSSLGLASEAADGSDWLLATGND3.31-bND
AP-2 peptides
AP2 pep QGDLLGDLLNLDLGPPVNVPQ0.30.443  ± 6.0 (28)
AP2 pep ΔPVN QGDLLGDLLNLDLGP AAAVPQ0.20.449  ± 14 (4)
AP2 pep ΔNLD QGDLLGDLLAAALGPPVNVPQ0.70.943  ± 11 (4)
AP2 pep ΔDLL QGDLLG AAANLDLGPPVNVPQ281-b2.51-b4.0  ± 2.6 (7)
AP2 pep D/L swap QG LDDG LDDN DLDGPPVNVPQ141-b501-b0 (3)
  • F1-a Numbers in parentheses indicate the number of experiments performed.

  • F1-b Because inhibition was <50% at 1 mm, these values are extrapolations.

  • F1-c ND, Not determined.