Table 1.

Identification of products from insulysin cleavage of Aβ1–40

Peak no.1–40 FragmentSequence
1  1–14DAEFRHDSGYEVHH
2  1–13DAEFRHDSGYEVH
3  1–18DAEFRHDSGYEVHHQKLV
4  1–19DAEFRHDSGYEVHHQKLVF
5  1–28DAEFRHDSGYEVHHQKLVFFAEDVGSNK
6  1–20DAEFRHDSGYEVHHQKLVFF
720–40FAEDVGSNKGAIIGLMVGGVV
829–40GAIIGLMVGGVV
921–40AEDVGSNKGAIIGLMVGGVV
1019–40FFAEDVGSNKGAIIGLMVGGVV
1115–40QKLVFFAEDVGSNKGAIIGLMVGGVV
  • The peaks from the HPLC chromatogram shown in Figure 2 were collected and analyzed by MALDI-TOF. Product peaks are labeled sequentially as derived from HPLC (shown in Fig. 2).