Table 1.

Targeting of the chimeras in LLC-PK1 cells and neurons

Embedded Image
  • Schematic diagrams of 5-HT1AR/5-HT1BR chimeras primary structures with 5-HT1AR sequence shown in black and 5-HT1BR sequence shown in gray. Chimeras A–F were previously constructed by combining three cassettes corresponding to (1) the first five TM domains, (2) the third intracellular domain, and (3) the last two TM domains and the cytoplasmic tail (Darmon et al., 1998). The truncated receptors were constructed by replacing the triplet of the amino acid indicated in the truncated receptor name by a stop codon. The chimeras 1ActB and 1BctA correspond to the entire receptors in which the small cytoplasmic tails have been exchanged between the two receptors. The targeting is resumed as it appeared predominantly in: LLC-PK1 cells, apical, basolateral, or Golgi; ER, endoplasmic reticulum; nf, nonfunctional, i.e., do not yield either stable or transient transfection. For neurons, somatodendritic means that the localization was restricted to the soma and dendrites, and axonal means that in addition to dendrites, the axon was labeled.