List of the RRL/EGL clones
| Embryo1-a | Inject (stage)1-b | Harvest1-c | RRL/EGL clone1-d | Origin1-e | No. Hb sib (cell type)1-f | Hb sib location1-g | No. unrelated clones1-h |
|---|---|---|---|---|---|---|---|
| JZ-2 | 10 | E8 | JZ2-10-3 | Medial | 3 picks (u) | LC, PR | Nil |
| JZ-10 | 10 | E8 | JZ10-4 | Medial | 6 picks (n, m, u) | LC, PR | 4 Hb |
| E8 | JZ10-19 | Medial | 2 picks (n, m, u) | LC, PR | |||
| JZ-5 | 10 | E8 | JZ5-1 | NF | 3 picks (n, u) and − area | PR | 1 Hb |
| E8 | JZ5-2 | NF | 3 picks (n, u) | PR, other | |||
| LC5-5 | 11 | E8–E9 | LC5-5-9-1 | Midline | 2 picks (n) | Isth | 7Hb, 1Hb + DCN, 1 DCN, 4Cb |
| JL36-13 | 10 | E9 | JL36-13-6-3 | Lateral | 8 picks (n, m, u) | VN, LC? | Nil |
| JL39-2 | 12 | E9.5 | JL39-2-9D-13 | Lateral | None | NA | 3 Cb |
| JL32-26 | 8 | E10 | JL32-26-11 | Lateral | 1 pick & − area | PR | 5 Hb |
| JL33-30 | 10 | E11–E12 | JL33-30-3-8 | Lateral | 3 picks (n, u) | LC, PR | 1 Isth, 1 Hb |
| JL33-42 | 10 | E11–E12 | JL33-42-4-16 | Lateral | None | NA | 1 Hb + DCN |
| JL40-12 | 12 | E15 | JL40-12-6B-7 | NF | None | NA | 2 Hb, 1 Cb glia |
| JL40-12-6B-17 | NF | None | NA | ||||
| JG24-4 | 12 | E18 | JG24-4-3-8 | NF | None | NA | 8 Pkj, 2 P + G, 1 DCN, 2 − |
| E18 | JG24-4-4/5-27 | NF | None | NA | |||
| JG24-8 | 12 | E18 | JG24-8-4-2 | NF | None | NA | 1 P + G, 2 Cb glia |
| JL32-7 | 10 | E18 | JL32-7-9-2 | NF | None | NA | 2 Hb |
| JL35-4 | 10 | E18 | JL35-4-3-1 | NF | 1 pick (n) | PIN | 4 Hb, 2 (−) |
| JL35-17 | 10 | E18 | JL35-17-9-1 | NF | 1 pick (n) | PIN | 1 Isth, 1 Hb, 1 Cb glia |
| JL35-17-9-20 | NF | None | NA |
The list shows all of the RRL/EGL clones in this study, as defined by PCR sequencing of the CHAPOL retroviral genome (see Materials and Methods).
↵F1-a Number assigned to the embryo.
↵F1-b HH stage of retroviral injection.
↵F1-c Embryonic day of harvest.
↵F1-d Number of the RRL/EGL clone. The RRL/EGL clones that contained progeny outside the cerebellum proper are shown in bold type.
↵F1-e The origin of a given clone is designated according the relative position along the width of the RRL, as midline, medial, or lateral. When no discernible origin in the RRL or the posterior margin of the cerebellum was observed, either because of a loss of tissue or because of maturity of the clone, the origin of the clone is designated NF (not found).
↵F1-f Number of cell picks in the hindbrain or isthmus region that were derived from an RRL/EGL clone with the tentative identity of the cell type(s) in the parenthesis. n, Neuron; m, migrating cell; u, unidentified; −, AP-negative pick.
↵F1-g Tentative localization of the RRL/EGL progeny in the hindbrain or isthmus region. Isth, Isthmus; LC, locus ceruleus; PIN, parvocellular isthmic nucleus; PR, pontine reticular formation; VN, vestibular nuclei; NA, not applicable.
↵F1-h Clones not lineally related to the RRL/EGL clone. Cb, Cerebellar VZ clone; Cb glia, cerebellar glial clone; P, Purkinje cell clone; P + G, Purkinje cell and glial clone; DCN, clone in the deep cerebellar nuclei; Hb, hindbrain-restricted clone; Isth, isthmus-restricted clone; −, AP-negative tissue pick. It should be noted that two clones were found to contain both neurons in the deep cerebellar nuclei and neurons in the vestibular nuclei in the hindbrain, thus designated Hb + DCN.