NR-ISH probe information
| Probe | Accession number | Primers | Position/size | % Identity/gaps2-a | ||
|---|---|---|---|---|---|---|
| eag1 | Z34264 | EcoRI restriction fragment from partial eag1 clone from phage screening | 2175 | w/Eag2 | 57 /6 | |
| EcoRI @ pos 2175 and polylinker EcoRI from pBluescript | 3087 | 912 bp | ||||
| eag2 | AF185637 | Forward: CGGAAGGTTTT(CT)(AG)A(N)GA(AG)CA(CT)C | 1849 | w/Eag1 | 64 /5 | |
| Reverse: CTGCTCGGG(TGA)AT(TGCA)GG(GA)TA(GA)AA | 2937 | 1089 bp | ||||
| erg1 | Z96106 | Forward: GACCTGCA(CT)AAGAT(CT)CA(GT)CGAG [ERGfam] | 2491 | w/Erg2 | 47 /7 | |
| Reverse: GGGAAACCTGAGAAAGCGAGT | 3349 | 858 bp | w/Erg3 | 42 /7 | ||
| erg1b | Z96106 | Forward: GAGCTGCTTCCTGTGTTTGG | 309 | w/Erg2 | 36 /18 | |
| Reverse: CTATGATTTCCCGGTCACTG | 1084 | 776 bp | w/Erg3 | 52 /12 | ||
| erg2 | AF016192 | Forward: [ERGfam] | 2074 | w/Erg1 | 40 /7 | |
| Reverse: CCTGTAAGCTACCTCTGAGCA | 3137 | 1063 bp | w/Erg3 | 38 /6 | ||
| erg3 | AF016191 | Forward: [ERGfam] | 2676 | w/Erg1 | 45 /6 | |
| Reverse: GAGACCCAAGATCCCTACAGT | 3731 | 1055 bp | w/Erg2 | 38 /6 | ||
| elk1 | AF061957 | Forward: GATCGT(GAC)GATGG(AC)ATTGA(AG)GA [ELKfam] | 2490 | w/Elk2 | 36 /9 | |
| Reverse: CAGTATAGAGGTGGCTCTGC | 3521 | 1031 bp | w/Elk3 | 36 /8 | ||
| elk2 | AJ007627 | Forward: [ELKfam] | 2500 | w/Elk1 | 35 /9 | |
| Reverse: GACAGAGGACAGTGGAGATG | 3523 | 1023 bp | w/Elk3 | 44 /7 | ||
| elk3 | AJ007628 | Forward: [ELKfam] | 2572 | w/Elk1 | 36 /8 | |
| Reverse: GAATGCTTTGAGCTGCTGGC | 3514 | 942 bp | w/Elk2 | 45 /7 | ||
| Kcnq2 | AF087453 | Forward: TCGATGACAGCCCAAGCAAG | 2916 | w/Kcnq3 | 46 /8 | |
| Reverse: CAACCCACACTACTCTATGC | 4221 | 1305 bp | w/Kcnq4 | 38 /36 | ||
| w/Kcnq5 | 44 /4 | |||||
| Kcnq2b | AF087453 | Forward: CGCAAGCTGCAGAATTTCCT | 1774 | w/Kcnq3 | 64 /5 | |
| Reverse: GTAGGTGTCGAAGTGGTCAT | 2344 | 570 bp | w/Kcnq4 | 73 /0 | ||
| w/Kcnq5 | 68 /0 | |||||
| Kcnq3 | AF091247 | Forward: GATGCCATAGAAGAAAGCCC | 1326 | w/Kcnq2 | 45 /9 | |
| Reverse: CACATGAGTCCAGAAGAGTC | 2247 | 921 bp | w/Kcnq4 | 42 /14 | ||
| w/Kcnq5 | 42 /12 | |||||
↵F2-a Identity to closest relatives. Full-length genes were first aligned using Clustal W. Matching residues and residues aligning with gaps within the probe region were then counted and divided by probe length for percentage.