Table 1.

Summary of GABA and flurazepam concentration–response data from cysteine mutant and wild-type α1β2γ2 GABAAreceptors

ReceptorGABAFlurazepam
EC50m)nHnEC50mut/EC50 αβγEC50(nm)nHnEC50mut/EC50 αβγ
αβγ18  ± 4.71.7  ± 0.331.0250  ± 491.2  ± 0.131.0
αβγY72C70  ± 121-1601.3  ± 0.233.9270  ± 641.3  ± 0.331.1
αβγT73C20  ± 5.61.6  ± 0.331.1160  ± 151.8  ± 0.330.6
αβγI74C30  ± 4.51.2  ± 0.131.780  ± 122.9  ± 1.230.3
αβγD75C39  ± 4.91-1601.5  ± 0.332.24700  ± 13001-1601.6  ± 0.6318.8
αβγI76C30  ± 131.4  ± 0.531.72600  ± 12001-1602.4  ± 0.7310.4
αβγF77C24  ± 111.0  ± 0.331.3  >10,0003
αβγF78C33  ± 5.8*1.3  ± 0.241.8130  ± 351.8  ± 0.330.5
αβγA79C30  ± 121.4  ± 0.391.7310  ± 451.4  ± 0.331.2
αβγQ80C9.0  ± 2.51.6  ± 0.430.5260  ± 281.0  ± 0.131.0
αβγT81C15  ± 1.61.5  ± 0.140.8350  ± 1701.5  ± 0.231.4
αβγW82CNo expressionNo expression
αβγY83C10  ± 1.91.6  ± 0.230.6170  ± 171.1  ± 0.240.7
  • Data represent mean ± SD values. n, Number of independent experiments; nH, calculated Hill coefficient.

  • * ,

  • F1-160 Indicate values significantly different from wild-type receptors, withp < 0.05 and p < 0.01, respectively.