Table 2.

Effects of actin depolymerization on PSD95- and Bassoon-labeled puncta

Neuron age (days in vitro)PSD-95 puncta/100 μmPSD-95 colocalized with synaptophysin on dendrites (%)Number of nonsynaptic PSD-95 puncta in soma
CONPost-LATCONPost-LATCON   LAT
5–615.2  ± 1.67.6  ± 0.8*48.8NA0.5  ± 0.56.0  ± 2.0
12–1348.8  ± 3.933.2  ± 2.3*93.477.7*1.9  ± 0.68.6  ± 2.4*
Bas puncta/100 μmBas puncta area (μm2) Bas intensity (% control)
CONPost-LATCONPost-LATPost-LAT
5–630.4  ± 1111.4  ± 3.4*0.11  ± 0.010.11  ± 0.0195  ± 0.03
12–1386.2  ± 5.562.6  ± 3.60.30  ± 0.010.21  ± 0.012-16086  ± 2.3*
  • CON, Control; post-LAT, after latrunculin A; Bas, Bassoon. Numbers are mean ± SEM.

  • * p < 0.002;

  • F2-160 p = 0.019.