Changes in Y2 and Y1 receptor binding and in lengths of NPY immunoreactive fibers in TLE specimen with hippocampal sclerosis
| % of control (mean ± SEM) | |||
|---|---|---|---|
| Y2 binding | Y1 binding | Length of NPY-IR fibers | |
| Specimens with hippocampal sclerosis | |||
| Dentate gyrus | |||
| Hilus | 148 ± 9.72-160 | n.d. | 286 ± 47.32-160 |
| Molecular layer | 71 ± 18.3 | 38 ± 3.52-160 | 1058 ± 2012-160 |
| CA3 | |||
| Stratum lucidum | n.d. | n.d. | 519 ± 1232-160 |
| Stratum radiatum | 146 ± 8.42-160 | n.d. | 615 ± 1122-160 |
| CA1 | |||
| Stratum radiatum | 143 ± 10.32-160 | n.d. | 215 ± 1382-160 |
| Stratum lacunosum moleculare | 70 ± 7.2* | n.d. | 269 ± 1282-160 |
| Subiculum | 127 ± 10.1* | n.d. | 690 ± 1522-160 |
| Non-sclerotic specimens | |||
| Dentate gyrus | |||
| Hilus | 112 ± 9.5 | n.d. | 179 ± 58 |
| Molecular layer | 73 ± 19.4 | 88 ± 7.7 | 162 ± 43 |
| CA3 | |||
| Stratum lucidum | n.d. | n.d. | 232 ± 68 |
| Stratum radiatum | 104 ± 8.0 | n.d. | 213 ± 71 |
| CA1 | |||
| Stratum radiatum | 109 ± 8.5 | n.d. | 202 ± 80 |
| Stratum lacunosum moleculare | 79 ± 11.8 | n.d. | 152 ± 61 |
| Subiculum | 88† | n.d. | 459 ± 126* |
Y2 and Y1 receptor binding was determined by receptor autoradiography with [125I]PYY3–36 and [125I][Pro34]PYY, respectively, and is expressed as percentage of controls. The following quantitative data (femtomoles per milligram of wet tissue weight ± SEM) were obtained in autopsies for [125I]PYY3–36binding in the hilus, 2.3 ± 0.13; molecular layer, 0.85 ± 0.19; stratum radiatum CA3, 2.3 ± 0.12; stratum radiatum CA1 2.0 ± 0.11; stratum lacunosum moleculare CA1, 0.18 ± 0.02; and for [125I][Pro34]PYY in the hilus, 0.12 ± 0.01, and molecular layer, 0.37 ± 0.02. Length of NPY immunoreactive fibers was determined in arbitrary units and is expressed as percentage of controls. Note that Y2 binding was increased in most areas but decreased in the molecular layer of the dentate gyrus and in the stratum lacunosum CA1. Note marked increases in NPY immunoreactive fibers (data for the outer molecular layer are shown; fiber length in the inner molecular layer was increased by 719 ± 179%). Receptor autoradiography was performed in 17 sclerotic, 3 nonsclerotic, and 9 autopsy specimens. NPY immunocytochemistry was performed in 32 sclerotic, 4 nonsclerotic, and 16 autopsy specimens.
↵† Binding in the subiculum was detectable in two specimens only. Statistical analysis was performed by ANOVA using a Dunnett post test. n.d., Not determined.
↵* p< 0.05;
↵F2-160 p < 0.01 versus controls.