5-HT1A receptor-mediated increase in [35S]GTP-γ-S binding to the dorsal raphe nucleus and the hippocampus of NK1−/− mutants compared with NK1+/+ wild-type mice
| [35S]GTP-γ-S binding (%) | ||
|---|---|---|
| DRN | Hippocampus | |
| NK1+/+ | 100 ± 9 | 100 ± 10 |
| NK1−/− | 59 ± 3* (−41%) | 89 ± 19ns (−11%) |
Results, expressed as percentage of NK1+/+ values, are the means ± SEM of independent determinations in six to seven mice per group. 5-HT1A receptor-mediated increase in [35S]GTP-γ-S binding attributable to 5-CT (10 μm) reached +131% over baseline in the DRN and +201% in the hippocampus of brain sections from NK1+/+ mice. ns, Nonsignificant;
↵* p < 0.05 as compared with NK1+/+ mice (unpaired Student's t test).