Table 1.

Conditional rescue of leoP1375 homozygous lethality by isoform-specific transgenes

Transformant line% Rescue at 18°C% Rescue at RT% Rescue by HS
LI-2.110.2 ± 6.2100100
LI-2.721.7 ± 7.5100100
LI-4.10028.6 ± 4.8
LI-4.3**3.5 ± 1.55.8 ± 2.480.4 ± 5.6
LII-1.2015.2 ± 3.147.4 ± 3.9
LII-3.0**0021.3 ± 5.2
LII-4.26.8 ± 2.523.8 ± 3.666.7 ± 4.6
LII-8.1016.8 ± 3.746.7 ± 4.5100
LII-12.006.1 ± 3.812.9 ± 4.1
  • Male and female animals of the genotypesleoP1375 /CyO;LEOI (LI) andleoP1375 /CyO;LEOII (LII) were mated, and progeny was reared at 18°C, 23 ± 2°C (RT), or under two 30 minute daily inductions (24–36.5 ± 0.5°C heat shocks) throughout development until adult progeny emerged. Percentage of rescue represents the fraction of obtained over expected adultleoP1375 homozygotes.