Relative current amplitudes of coexpressed GluR7 mutants and chimeras between GluR6 and GluR7
| Clone | KA current [%] | Glu current [%] | n |
|---|---|---|---|
| GluR6(Q) + H2O | 100.0 ± 9.7 | 100.0 ± 8.6 | 7 |
| GluR7(insR651a/K652R/P653Q) + GluR7(K758N) | 0.2 ± 0.1 | 1.9 ± 1.0 | 5 |
| GluR7(insR651a/K652R/P653Q) + GluR7(I731M) | 0.8 ± 0.3 | 2.1 ± 0.7 | 5 |
| GluR7(K626E) + GluR7(I731M) | 0.0 ± 0.0 | 0.0 ± 0.0 | 3 |
| GluR7(I731M) + GluR7(K758N) | 0.09 ± 0.04 | 0.90 ± 0.04 | 6 |
| GluR7(insR651a/K652R/P653Q) + GluR7–GluR6L3C/FC | 14.9 ± 5.8 | 9.5 ± 3.6 | 4 |
| GluR7(K6262E) + GluR7-GluR6L3C/FC | 6.9 ± 4.0 | 6.7 ± 4.5 | 4 |
| GluR7–GluR6L3N + GluR7–GluR6L3C/FC | 0.8 ± 0.2 | 0.8 ± 0.2 | 3 |
Receptors expressed in Xenopus oocytes were probed with 300 μm glutamate and 300 μm kainate as agonists. All currents were measured after 8 min of pretreatment of oocytes with ConA to minimize desensitization. Relative currents were calculated by taking GluR6 currents of the same batch as 100%. Data are shown as means ± SEM.