Table 2.

Effects of chelerythrine on the METH-induced decrease in [3H]DA uptake

Treatment[3H]DA uptake ± SEM
Control0.64  ± 0.06
10 μm METH0.31  ± 0.03*
0.1 μm chelerythrine0.61  ± 0.09
0.1 μm chelerythrine/10 μmMETH0.29  ± 0.02*
1 μmchelerythrine0.57  ± 0.08
1 μmchelerythrine/10 μm METH0.27  ± 0.04*
  • Striatal synaptosomes were pretreated with 1 or 0.1 μm chelerythrine for 5 min and then preincubated with 10 μm METH or assay buffer for 30 min at 37°C. Before assaying [3H]DA influx, synaptosomal preparations were washed two extra times, as described in Materials and Methods. Values represent means and 1 SEM of determinations from three independent experiments. Values for [3H]DA uptake represent femtomoles per microgram of protein.

  • * Values different from respective control groups (p ≤ 0.05).