Effect of GLT-1 and EAAC1 knockdown on the infarct volume after transient MCAO
| ICV infusion | n | Cortex (mm3) | Striatum (mm3) | Total (mm3) |
|---|---|---|---|---|
| None | 6 | 172 ± 27 | 31 ± 5 | 203 ± 28 |
| aCSF | 5 | 175 ± 36 | 37 ± 7 | 208 ± 39 |
| GLT-1 sense | 5 | 167 ± 34 | 34 ± 9 | 204 ± 41 |
| GLT-1 random | 4 | 164 ± 33 | 32 ± 6 | 199 ± 47 |
| GLT-1 antisense | 8 | 246 ± 501-a,1-b | 48 ± 71-a,1-b | 294 ± 521-a,1-b |
| EAAC1 sense | 4 | 186 ± 44 | 33 ± 10 | 222 ± 47 |
| EAAC1 random | 4 | 175 ± 49 | 36 ± 9 | 210 ± 52 |
| EAAC1 antisense | 6 | 201 ± 46 | 39 ± 7 | 240 ± 50 |
Values are mean ± SD. The MCAO duration was 1 hr in all cases. Ischemic injury volumes were computed by volumetric analysis of the thionine-stained coronal sections using the NIH Image program. aCSF, Artificial CSF; ICV, intracerebroventricular.
↵F1-a p < 0.05, compared with the None or aCSF group (one-way ANOVA followed by Dunnet's multiple comparisons post test).
↵F1-b p < 0.05, compared with the respective sense or random group (ANOVA followed by Tukey-Kramer multiple comparisons post test).