Table 1.

Kinetic properties of wild-type and mutant AMPARs

Receptor	τ (msec)	τ_rec (msec)	τ_deact(msec)	I_peak (pA)	I_ss (% I_peak)	n
Chimera 1	1.53 ± 0.03^1-165			30 ± 20	7 ± 1	5
Chimera 2	2.12 ± 0.05^1-165			78 ± 37	6 ± 2	5
Chimera 3	1.57 ± 0.11^1-165			107 ± 17	5 ± 2	7
Chimera 4	4.03 ± 0.21^###			141 ± 50	9 ± 2	6
Chimera 5	3.82 ± 0.15^###			109 ± 36	7 ± 2	5
Chimera 6	2.54 ± 0.09^†††			59 ± 18	8 ± 2	10
Chimera 7	2.51 ± 0.16^†††			49 ± 10	5 ± 1	6
GluR1_o	3.84 ± 0.12^###	155 ± 16	0.99 ± 0.11	60 ± 13	8 ± 1	13,10,10
E665D	3.63 ± 0.22^###			42 ± 10	9 ± 2	6
A666S	3.58 ± 0.30^###			68 ± 36	7 ± 1	5
F681Y	3.71 ± 0.32^###			30 ± 9	6 ± 1	5
T686S	3.57 ± 0.12^###			40 ± 11	7 ± 2	6
V697T	3.57 ± 0.15^###			140 ± 23	6 ± 1	9
R698K	3.86 ± 0.15^###			82 ± 17	5 ± 1	5
E701A	3.76 ± 0.35^###			19 ± 8	9 ± 1	7
E702D	3.83 ± 0.30^###			102 ± 25	6 ± 2	7
M704V	3.83 ± 0.46^###			94 ± 83	5 ± 2	5
I705A	3.87 ± 0.44^###			181 ± 27	9 ± 1	7
Y714F	3.04 ± 0.21^###,^1-160			49 ± 19	6 ± 2	7
Y716F	2.45 ± 0.35^1-165^{,^##}	117 ± 10	0.94 ± 0.11	14 ± 2	9 ± 1	5,5,7
I748V	3.92 ± 0.13^###			54 ± 13	9 ± 1	6
R757G	2.55 ± 0.10^†††	52 ± 11^*	1.13 ± 0.06	119 ± 25	5 ± 1	19,5,4
P759A	3.75 ± 0.30^###			86 ± 15	7 ± 1	14
Y714F + Y716F	2.89 ± 0.17^†††	149 ± 24	1.02 ± 0.08	112 ± 25	9 ± 2	14,5,9
Y714F + R757G	2.49 ± 0.12^†††	60 ± 6^*	1.02 ± 0.20	128 ± 60	8 ± 2	10,5,4
Y716F + R757G	1.75 ± 0.07^1-165	56 ± 19^*	1.12 ± 0.12	54 ± 14	7 ± 1	7,7,4
Y714F + Y716F + R757G	1.67 ± 0.05^1-165	90 ± 9^*	1.02 ± 0.10	102 ± 30	9 ± 1	21,8,9
GluR3_o	1.49 ± 0.06^1-165	142 ± 25	1.05 ± 0.09	28 ± 5	8 ± 2	16,5,6
F728Y	1.19 ± 0.04^1-165^{,^‡}			107 ± 29	8 ± 2	5
F728Y + G769R	1.84 ± 0.10^1-165^{,^‡,^§§}			138 ± 29	6 ± 1	10
GluR4c_o	1.17 ± 0.08^1-165			120 ± 34	5 ± 1	9
F724Y	0.95 ± 0.03^1-165^{,^1-159}			61 ± 33	6 ± 1	6
G765R	1.77 ± 0.26^1-165^{,^1-153,}			33 ± 17	7 ± 2	6
F724Y + G765R	1.64 ± 0.06^1-165^,			78 ± 19	5 ± 2	4

All receptors were expressed in Xenopus laevisoocytes, and currents were recorded in outside-out patch-clamp configuration after 10 mm l-glutamate stimulation.^{*,#,†,§,‡}p < 0.05;
↵F1-160 ^,##,§§,p < 0.01;
↵F1-165 ^{,###,†††,}p < 0.001.
↵* versus GluR1_o,
↵F1-159 versus GluR3_o,
† versus GluR1_o and GluR3_o,
↵F1-153 versus GluR4c_o,
F1-226 versus (F724Y)GluR4c_o.
n = number of eggs recorded for each parameter (τ, τ_rec, τ_deact).