Table 1.

Percentage aggregation of Cx- and mock-transfected C6 glioma cells

Percentage of cells in aggregatesPercentage of donor cells transferring dyeMean number of receiving cells per donor cellCoupling index
C6-Cx4372  ± 449  ± 414  ± 27  ± 0.07
C6-Cx4065  ± 355  ± 29  ± 15  ± 0.2
C6-Cx3254  ± 3*89  ± 321  ± 318  ± 0.1
C6-mock 115  ± 4*13  ± 1.21.1  = 0.30.14  ± 0.04*
C6-mock 213  ± 3*0.8  ± 0.21.1  ± 0.20.01  ± 0.005*
C6-mock 310  ± 4*18  ± 1.21.2  ± 0.20.2  ± 0.1*
C6-WT18  ± 3*27  ± 23  ± 0.10.8  ± 0.2*
  • Quantitative analysis of short-term aggregation assays. This table lists the percentage of Cx- and mock-transfected C6 cells that form aggregates in suspension assay. Dye transfer assays ofGoldberg et al. (1995) were used as described in Materials and Methods. All assays were performed in a minimum of three independent runs (range, 3–16; mean, 9).

  • * , Significantly different from C6-Cx43 cells (p < 0.05). ANOVA for group comparison with post hoc t test is used to establish significant difference between the groups. All numbers indicate ± SEM.