Table 2.

The synergistic effect of presynaptic and postsynaptic NarpN on synapse formation in cultured spinal neurons

Presynaptic and postsynaptic construct
ControlNarpN
GluR1 clusters/contact (n = 4)0.46  ± 0.050.16  ± 0.04*
Synaptophysin clusters/contact (n = 4)0.81  ± 0.060.55  ± 0.06*
GAD clusters/contact (n = 3)0.17  ± 0.040.19  ± 0.03
Gephyrin clusters/contact (n = 4)0.24  ± 0.050.31  ± 0.04
Narp clusters/contact (n = 4)0.47  ± 0.060.18  ± 0.052-160
BNP1 clusters/contact (n = 4)0.43  ± 0.070.24  ± 0.04*
Synapsin1 clusters/contact (n = 4)0.75  ± 0.080.57  ± 0.05*
  • Sites of contact between transfected axons and dendrites were scored as positive or negative for the indicated immunohistochemically detected protein. All numbers represent the averaged mean ± SD of the mean for the indicated number of transfections.

  • * p < 0.02 compared with control and with presynaptic NarpN alone.

  • F2-160 p < 0.01 (paired mean analysis) compared with control and with presynaptic NarpN alone.