Table 1.

Role of F-actin in ephrin-A2 induced endocytosis

TreatmentNumber of dextran-labeled vesicles (n)
Vehicle (DMSO)4.9  ± 0.5 (84)
Cytochalasin D5.5  ± 0.6 (82)
Latrunculin-A4.3  ± 0.5 (89)
Vehicle + ephrin-A211.1  ± 0.9 (76)*
Cytochalasin D + ephrin-A210.1  ± 0.8 (87)*
Latrunculin-A + ephrin-A29.1  ± 0.8 (80)*
  • Retinal growth cone collapse induced by F-actin depolymerizing drugs did not increase endocytosis compared with vehicle alone (p > 0.05). Addition of ephrin-A2 resulted in a significant increase in endocytosis in all conditions examined.

  • * Significant differences compared with identical cultures without ephrin (p < 0.001).