Table 1.

Compositions of pipette solutions for dialysis

Calculated [Ca2+]∼0.1 nm50 nm100 nm170 nm300 nm500 nm170 nm (ATP-γ-S)
KCl20202020202020
KAsp40404040404040
HEPES40404040404040
GTP0.10.10.10.10.10.10.1
Na2ATP2.02.02.02.02.02.0
Li4ATP-γ-S2.0
MgCl21.01.01.01.01.01.01.0
EGTA202020202020
BAPTA10
CaCl2081214161714
Indo-10.10.10.10.10.10.10.1
pCa∼107.37.06.86.56.36.8
  • The concentration of all ingredients is listed in millimolar. All solutions were adjusted to pH 7.4 by KOH.