All cells were loaded for 20 min with 10 μmCM-H₂DCFDA. They were then transferred to L-15 medium and exposed to H₂O₂. Intensity is shown as fold change from CM-H₂DCFDA intensity measured in NGF-maintainedbax^+/+ cells that were plated at the same time.n = 26-112 cells from two or three separate platings. There were no significant differences in dye intensities of cells treated with the same concentrations of H₂O₂(p > 0.05). These data demonstrate that the different Bax levels (±NGF) in the three genotypes did not interfere with the concentration of CM-H₂DCFDA loaded into cells or with the ability of CM-H₂DCFDA to be oxidized, and the compounds tested did not interfere with dye loading or ability of the dye to be oxidized. All NGF deprivation (−NGF) was for 18 hr.