Table 2.

Phenotypic characterization of differentiated clonal SN progenitor cells propagated in the presence of FGF2 or FGF8

Neurons onlyGlia onlyBipotentNo markers
FGF224.7%4.7%4.7%65.9%
FGF840%13.3%11.1%35.6%
  • SN progenitor cells were infected with low-titer GFP retrovirus. The progeny of single infected cells was closely monitored. Cells were allowed to proliferate for 7 d after infection in the presence of FGF2 or FGF8 and differentiated for 7 d in the presence of retinoic acid and FBS. GFP-positive clones were analyzed for the expression of lineage-associated markers for neurons (β-tubulin III) and astrocytes (GFAP). Neurons only, Percentage of clones that contained only β-tubulin III-positive cells; Glia only, percentage of clones that contained only GFAP-positive cells; Bipotent, percentage of clones that contained β-tubulin III-positive and GFAP-positive cells; No markers, percentage of clones that were negative for glial and neuronal markers.