Comparison of guidance defects
| unc-6 transgene | Genetic background | Dorsal guidance (% defective) | Ventral guidance (% defective) | |||||
|---|---|---|---|---|---|---|---|---|
| Axon guidance | Cell guidance | Axon guidance | Cell guidance | |||||
| DA, DB1-a | SDQR1-b | DTC1-canterior | DTC1-cposterior | PVCL1-d | AVM1-e | EGL1-f | ||
| Wild type | 0 | 2 ± 1 | 0 | 0 | 0 | 0 | 0 | |
| unc-6(+) | unc-6(ev400) | ND | 3 ± 1 | 0 | 0 | ND | 0 | 0 |
| unc-6(ev400) | 98 ± 2 | 91 ± 3 | 38 ± 1 | 62 ± 3 | 39 ± 2 | 27 ± 2 | 55 ± 2 | |
| unc-40(e1430) | 25 ± 1 | 57 ± 4 | 9 ± 3 | 25 ± 2 | 26 ± 1 | 15 ± 2 | 49 ± 1 | |
| unc-5(e53) | 99 ± 1 | 95 ± 2 | 40 ± 3 | 64 ± 1 | 0 | 0 | 0 | |
| unc-40(e1430); unc-6(ev400) | 99 ± 1 | 94 ± 3 | 43 ± 3 | 65 ± 4 | 30 ± 2 | 25 ± 3 | 73 ± 3 | |
| unc-5(e53); unc-6(ev400) | 99 ± 1 | 93 ± 4 | 45 ± 3 | 73 ± 3 | 35 ± 2 | 25 ± 1 | 58 ± 2 | |
| unc-6ΔV-1 | unc-6(ev400) | 0 | 3 ± 1 | 0 | 0 | 0 | 0 | 0 |
| unc-6ΔV-2 | unc-6(ev400) | 99 ± 2 | 85 ± 3 | 43 ± 2 | 61 ± 2 | 5 ± 2 | 14 ± 3 | 34 ± 1 |
| unc-6(rh204) | 94 ± 1 | 88 ± 4 | 40 ± 3 | 57 ± 5 | 0 | 11 ± 2 | 16 ± 2 | |
| unc-6V-1-3-3 | unc-6(ev400) | 98 ± 3 | 89 ± 2 | 40 ± 4 | 64 ± 4 | 13 ± 3 | 10 ± 1 | 37 ± 3 |
| unc-6ΔV-3 | unc-6(ev400) | 95 ± 2 | 89 ± 2 | 35 ± 2 | 65 ± 3 | 16 ± 2 | 8 ± 3 | 50 ± 3 |
| unc-6(e78) | 90 ± 2 | 80 ± 2 | 0 | 15 ± 1 | 0 | 0 | 10 ± 1 | |
| unc-6ΔC | unc-6(ev400) | 9 ± 2 | 56 ± 4 | 3 ± 3 | 13 ± 2 | 4 ± 1 | 18 ± 2 | 30 ± 2 |
↵F1-a The DA, DB motor neuronal cell bodies are located along the ventral nerve cord, and from each an axon migrates dorsally to the dorsal nerve cord.
↵F1-b The SDQR neuron cell body is located laterally, and the axon migrates dorsally to the dorsal sublateral nerve in the larva.
↵F1-c The hermaphrodite gonad is created by the migrations of the two distal tip cells, which at one point migrate dorsally. In the mutants, the distal tip cells may fail to migrate dorsally and instead migrate along the ventral muscle quadrant.
↵F1-d The PVCL neuron cell body is located posteriorly, and the axon migrates ventrally into the ventral nerve cord.
↵F1-e The AVM neuronal cell body is located laterally, and the axon migrates ventrally to the ventral nerve cord.
↵F1-f L4 stage hermaphrodites were placed individually onto seeded plates and examined at 12 hr intervals to determine whether any eggs were laid. Animals were scored as abnormal if they were incapable of egg laying. Three independently derivedunc-6 transgenic strains were counted for each phenotype.n = 100. Mean ± SEM.