Table 2.

Rates and extents of SERT mutant inactivation by MTSEA in membrane preparations

MutantIonRate constant K, min−1 · m−1Residual activity, %n
X8C-L 137CNa+111  ± 1712  ± 64
X8C-L 137CK+122  ± 99.7  ± 33
X8C-I 157CK+722  ± 772  ± 42
X8C-S 277CNa+3795  ± 15234.5  ± 22
X8C-357CNa+567  ± 2003.6  ± .63
X8C-A 441CNa+6716  ± 2342.2  ± .53
  • The data were calculated by fitting curves of inactivation versus MTSEA concentration. The calculated concentration giving half-maximal inactivation in 15 min was used to calculate the rate constant. Values are given with SDs, and n is the number of experiments that were used in the calculations.