Table 2.

Growth cone attraction induced by FLIP of caged taxol

Laser irradiationChemicals in bathCulture typesTuring angle (degree)*Extension (μm)*Turning scores (%)2-aNumber of cells examined
+0
Control 1+None6 hr−0.3  ± 3.619.1  ± 1.629422924
Control 2Caged taxol6 hr0.4  ± 3.910.2  ± 0.72-16042213628
Uncaging+Caged taxol6 hr11.7  ± 3.52-16010.2  ± 0.82-1607813923
  • * Values represent mean ± SEM.

  • F2-160 The turning angle was significantly different from both control groups (p < 0.01; Kolmogorov–Smirnov test). The presence of caged taxol in bath appeared to inhibit neurite extension (p < 0.01 when comparing with control 1).

  • F2-a Growth cone turning responses were scored as follows: +, growth cones showing positive turning toward the side of laser application (turning angle ≥5°); 0, growth cones showing no turning (turning angle <5°); −, growth cones turning away (turning angle ≤−5°).