Chemicals in pipette | Chemicals in bath | Pipette distance (μm) | Culture types | Turning angle (degree)3-150 | Extension (μm)3-150 | Turning scores (%)3-a | Number of cells examined | ||
---|---|---|---|---|---|---|---|---|---|
+ | 0 | − | |||||||
1% DMSO | None | 50 | 6 hr | −1.3 ± 2.5 | 14.0 ± 1.1 | 37 | 26 | 37 | 30 |
0.5 μm taxol | None | 50 | 6 hr | 3.8 ± 3.0 | 14.2 ± 1.1 | 52 | 16 | 32 | 25 |
5 μm taxol | None | 50 | 6 hr | 11.0 ± 2.23-160 | 17.3 ± 1.8 | 80 | 8 | 12 | 25 |
50 μm taxol | None | 50 | 6 hr | 11.0 ± 3.23-160 | 15.2 ± 1.5 | 62 | 21 | 17 | 24 |
5 μm taxol | 20 nm cytochalasin D | 50 | 6 hr | −0.1 ± 4.4 | 12.8 ± 1.4 | 41 | 24 | 35 | 17 |
5 μm taxol | 100 pg/ml toxin B | 50 | 6 hr | 0.5 ± 3.1 | 8.8 ± 3.53-160 | 44 | 22 | 34 | 18 |
10 μm nocodazole | None | 50 | 6 hr | −0.7 ± 2.7 | 12.3 ± 0.8 | 28 | 34 | 38 | 29 |
100 μm nocodazole | None | 50 | 6 hr | −11.4 ± 3.03-160 | 10.4 ± 0.93-160 | 14 | 25 | 61 | 36 |
↵F3-150 Values represent mean ± SEM.
↵F3-160 Significantly different from the control groups (p < 0.05; Kolmogorov–Smirnov test).
↵F3-a Growth cone turning responses were scored as follows: +, growth cones showing positive turning toward the pipette (turning angle ≥5°); 0, growth cones showing no turning (turning angle <5°); −, growth cones turning away (turning angle ≤−5°).