Lineage description | Output synapse distribution | Synapse count | Presynaptic arbor area | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Name | n(wt) | Figure | DV | CI | AP | ML | Compart | wt | kak | wt | kak |
VUM | 8 | 4B,B | X | − | − | − | |||||
MNB | 2 | 4A, 4F | X | − | 33 and 64 | 37 | 0.4 and 0.46 | 0.51 | |||
NB1-1 | 2 | − | X | − | 13 and 26 | 16 | 0.1 and 0.06 | 0.1 | |||
NB2-1 | 2 | 4C,C′ | X | X | + | − | − | ||||
NB3-1 | 2 | − | X | X | + | − | − | ||||
NB3-2 | 10 | − | X | + | 39 to 81 | 52 | 0.1 to 0.21 | 0.19 | |||
57.3 ± 13.72 | 0.14 ± 0.033 | ||||||||||
NB5-2 | 12 | 4G,H, 5D | X | X | + | 24–56 | 37 | 0.16 to 0.33 | 0.21 | ||
40.7 ± 10.35 | 0.25 ± 0.063 | ||||||||||
NB5-3 | 3 | − | X | X | + | − | − | ||||
NB6-1 | 4 | 4D,D′ | X | + | − | − | |||||
NB6-2 | 8 | 4E, E′, 5E | X | X | + | 46–80 | (40?) | − | (?) | ||
64 ± 12,86 | (Damage) | (Damage) | |||||||||
NB7-1 | 2 | 4F,F′ | X | + | − | − |
Lineage description: Name, name of neural precursor that the lineage is assigned to; n (wt), number of wild-type lineages obtained; Figure, reference to images shown in this paper. Output synapse distribution: output synapses show specific dorsoventral (DV), contralateral–ipsilateral (CI), anteroposterior (AP), or mediolateral (ML) distribution, or reveal (+)/lack (−) obvious compartmentalized distribution in individual neurites (Compart). Synapse count: Counts of individual Synapsin spots of wild-type (wt) or kakapo(kak) mutant cell lineages (in cases of n > 2: lowest to highest number; mean ± SD; Damage, synapse number in the kakapo mutant NB6-2 lineage might be low because of distortion of the preparation; see Fig. 5E). Counts were performed on serial confocal sections and restricted to defined areas of lineages in which spots could be reliably resolved. Defined areas were as follows: MNB, total; NB1-1, all posterior ipsilateral spots; NB3-2, total; NB5-2, all contralateral spots anterior of characteristic transverse projection (indicated by arrow in Figs. 4,G and H, and 5, A and D). Presynaptic arbor area, Areas covered by the counted Synapsin spots; values represent measured presynaptic area divided by the square of the neuropile width of each respective neuropile.