Table 2.

Quantitative analysis of the polarity of EAAT constructs in hippocampal neurons

ConstructCells (n)Presence in axonsPresence in dendritesAxon/dendrite ratio2-a
EAAT310++0.06  ± 0.02
E3Δ470–52411+++0.28  ± 0.03*
E3Δ498–52411+++0.29  ± 0.05*
E3(504–509AAAAAA)14+++0.26  ± 0.02*
EAAT210++++0.68  ± 0.09
E2-3(498–524)12+++0.31  ± 0.042-160
E2-3(498–524:504–506AAA)11++++0.82  ± 0.10
  • Expression of the EAAT constructs was analyzed at 11 din vitro.

  • F2-a Values are mean ± SEM.

  • * Significantly different from EAAT3;

  • F2-160 significantly different from EAAT2 (p < 0.05).