Comparison of the expression levels of dopaminergic markers in Cpu and SNc of α6−/− and Wt mice
| Wt (Cpu) | α6−/− (Cpu) | Wt (SNc) | α6−/− (SNc) | |
|---|---|---|---|---|
| Cell count | 82.6 ± 19.4 | 89.3 ± 14.4 | 163.7 ± 5.5 | 158.0 ± 5.0 |
| TH (ICC) | 0.039 ± 0.002 | 0.037 ± 0.002 | 0.26 ± 0.03 | 0.25 ± 0.03 |
| DAT (ICC) | 0.098 ± 0.002 | 0.100 ± 0.002 | 0.31 ± 0.02 | 0.32 ± 0.02 |
| TH (ISH) | ND | ND | 0.83 ± 0.02 | 0.82 ± 0.01 |
| D1 (ISH) | 0.57 ± 0.05 | 0.56 ± 0.02 | ND | ND |
| D2 (ISH) | 0.70 ± 0.01 | 0.68 ± 0.01 | 0.53 ± 0.01 | 0.53 ± 0.02 |
| PPE (ISH) | 0.62 ± 0.01 | 0.58 ± 0.02 | ND | ND |
| PPT (ISH) | 0.70 ± 0.02 | 0.70 ± 0.01 | ND | ND |
| CCK (ISH) | ND | ND | 0.33 ± 0.01 | 0.33 ± 0.01 |
| D1(LBA) | 1.06 ± 0.03 | 1.02 ± 0.04 | ND | ND |
| D2 (LBA) | 0.60 ± 0.02 | 0.58 ± 0.01 | 0.041 ± 0.001 | 0.044 ± 0.001 |
In situ hybridization (ISH), immunocytochemistry (ICC), and ligand binding autoradiography (LBA) were used to compare the levels of expression of various markers in Wt and α6−/− mice at the striatal and mesencephalic levels. Only the results of semiquantitative analysis in Cpu and SNc are presented here. Except for the cell number, each value represents the mean specific optical density ± SEM, obtained from eight animals of each genotype. Cell count was performed on TH-immunostained (SNc) or Nissl-stained (Cpu) sections. [3]SCH23390 and [3H]raclopride were used for the determination of D1 and D2 dopamine receptor levels (respectively). ND, Not detected.