Summary of dissection technique and culture conditions used for the comparison of adult subependymal and dentate gyrus primary cell isolates
| SE dissection | DG dissection | Gross HC dissection | |
|---|---|---|---|
| EGF + FGF2 + H + B27 (uncoated dishes) | + | − | + |
| 10% FBS; FGF2 + N2 (uncoated dishes; or polyornithine/laminin coated) | + | − | + |
| 10% FBS; FGF2 + N2 + AHP-conditioned media (uncoated dishes; or polyornithine/laminin coated) | + | − | + |
Summary table illustrating the various dissection techniques and culture conditions used for the comparison of primary adult subependymal and dentate gyrus cells. A simplified version of the results is also illustrated where “+” represents the isolation of a multipotent, self-renewing cell in the tissue cultures from the various experimental conditions. Importantly, cell viability in all culture conditions was high. SE, Subependyma; DG, dentate gyrus; HC, hippocampus; EGF, epidermal growth factor; FGF2, fibroblast growth factor 2; H, heparin; B27, B27 supplement; N2, N2 supplement; FBS, fetal bovine serum; AHP, adult hippocampal progenitor.