Summary of anatomical and physiological phenotypes induced by Robo, Robo2, and Robo3 expression in the GF
| Genotype1-a | Anatomy | Physiology | |||||||
|---|---|---|---|---|---|---|---|---|---|
| n | Lateral deflection (% GFs) | Lateral position (μm) | ben-like (% GFs) | n | Latency ± SEM (msec) | Normal synapse (% flies)1-b | Weak synapse (% flies)1-c | No synapse (% flies)1-d | |
| c17/+ | 50 | 0 | 1 | 0 | 17 | 0.91 ± 0.02 | 100 | 0 | 0 |
| c17/+;UAS-robo-myc/+ | 26 | 29 | 1.4 | 14 | 32 | 0.98 ± 0.11 | 80 | 22 | 0 |
| c17/+;(2×)UAS-robo/+ | 50 | 76 | 2.2 | 14 | 53 | 1.23 ± 0.15 | 68 | 30 | 2 |
| c17/+;UAS-robo2-myc/+ | 30 | 44 | 1.6 | 0 | 16 | 0.85 ± 0.03 | 100 | 0 | 0 |
| c17/+;UAS-robo3/+ | 16 | 57 | 1.9 | 0 | 11 | 0.9 ± 0.02 | 100 | 0 | 0 |
| c17/+;UAS-roboΔC2+3/+ | 39 | 13 | 1.1 | 0 | 50 | 0.89 ± 0.06 | 92 | 8 | 0 |
| A307/+ | 50 | 0 | 1 | 0 | 38 | 0.89 ± 0.02 | 100 | 0 | 0 |
| A307/+;UAS-robo-myc/+ | 33 | 100 | 3.3 | 27 | 22 | 1.81 ± 0.20 | 32 | 45 | 23 |
| A307/+;(2×)UAS-robo/+ | 55 | 100 | 3.6 | 26 | 25 | 1.55 ± 0.26 | 36 | 36 | 28 |
| A307/+;UAS-roboΔC/+ | 22 | 0 | 1 | 5 | 13 | 1.02 ± 0.02 | 77 | 23 | 0 |
| A307/+;UAS-roboΔC2+3/+ | 27 | 100 | 3.3 | 4 | 21 | 1.00 ± 0.10 | 60 | 31 | 9 |
| A307/+;UAS-robo2-myc/+ | 55 | 100 | 4.7 | 0 | 15 | 0.8 ± 0.03 | 100 | 0 | 0 |
| A307;UAS-robo2-myc +; UAS-robo2-myc | 20 | 100 | 5.0 | 0 | 13 | 0.92 ± 0.02 | 100 | 0 | 0 |
| A307/+;UAS-robo3/+ | 27 | 100 | 5.4 | 0 | 14 | 0.94 ± 0.08 | 86 | 14 | 0 |
| A307/+;UAS-comm | 20 | 0 | 1 | 0 | 11 | 0.81 ± 0.03 | 73 | 27 | 0 |
↵F1-a For anatomy, the flies also contained a UAS-lacZ either on X or on the second chromosome.
↵F1-b A normal synapse is defined as response latency ≤1 msec and follow stimuli up to 100 Hz.
↵F1-c A weak synapse is defined as response latency >1 msec or do not follow stimuli given at 100 Hz.
↵F1-d These animals showed no response to brain stimulation, but thoracic stimulation of these specimen revealed that the neuromuscular junction response is normal.