Summary of anatomical and physiological phenotypes induced by Robo, Robo2, and Robo3 presynaptic and postsynaptic expression in the GF and the TTMn
| Genotype3-a | Anatomy | Physiology | |||||||
|---|---|---|---|---|---|---|---|---|---|
| n | Lateral deflection (% GFs) | ben-like (% GFs) | Disrupted dendrites (% TTMs) | n | Latency ± SEM (msec) | Normal synapse (% flies)3-b | Weak synapse (% flies)3-c | No synapse (% flies)3-d | |
| A307/+, (2×)UAS-robo/+ | 55 | 100 | 26 | 25 | 1.55 ± 0.26 | 36 | 36 | 28 | |
| ShakB-Gal4/+, (2×)UAS-robo/+ | 21 | – | – | 100 | 14 | 2.09 ± 0.05 | 0 | 93 | 7 |
| ShakB-Gal4/A307, (2×)UAS-robo/+ | 30 | 100 | 46 | 100 | 23 | 1.64 ± 0.20 | 22 | 30 | 48 |
| A307/+,UAS-robo2-myc/+ | 55 | 100 | 0 | 15 | 0.80 ± 0.03 | 100 | 0 | 0 | |
| ShakB-Gal4/+,UAS-robo2-myc/+ | 20 | – | – | 0 | 14 | 0.93 ± 0.03 | 100 | 0 | 0 |
| ShakB-Gal4/A307,UAS-robo2-myc/+ | 17 | 100 | 0 | 0 | 19 | 0.95 ± 0.03 | 100 | 0 | 0 |
↵F3-a For anatomy, the flies also contained a UAS-lacZ either on X or on the second chromosome.
↵F3-b A normal synapse is defined as response latency ≤1 msec and follow stimuli up to 100 Hz.
↵F3-c A weak synapse is defined as response latency >1 msec or do not follow stimuli given at 100 Hz.
↵F3-d These animals showed no response to brain stimulation, but thoracic stimulation of these specimen revealed that the neuromuscular junction response is normal.