Inhibition of the recombinant NR1a/NR2B NMDA receptor by nucleotides and inhibitors of P2 receptors
| Compound | Inhibition (%) | n |
|---|---|---|
| ATP | 82 ± 4.2 | 6 |
| ADP | 17 ± 2.9 | 3 |
| AMP | 72 ± 8 | 4 |
| Adenosine | 2 ± 1.8 | 5 |
| GTP | 77 ± 6.4 | 3 |
| ITP | 69 ± 7.5 | 3 |
| CTP | 5 ± 3.5 | 4 |
| UTP | 3 ± 1.8 | 3 |
| 2MeSATP | 56 ± 8.1 | 3 |
| α,β-meATP | 38 ± 8.1 | 5 |
| α,β-meADP | 15 ± 4.7 | 3 |
| Suramin (100 μm) | 9 ± 3 | 4 |
| PPADS (100 μm) | 6 ± 3.6 | 5 |
| Reactive blue 2 (100 μm) | 46 ± 7.5 | 4 |
Inhibition was investigated after NR1a/NR2B cRNA injection into oocytes at NMDA and glycine concentrations of 20 and 10 μm, respectively. Values represent the percentage of current reduction determined at a holding potential of - 70 mV. Unless indicated otherwise, compounds were applied at a concentration of 500 μm. Values are means ± SEM of n determinations.