Effects of the group I mGluR agonist 3,5-DHPG on the release of [3H]ACh from human cortical synaptosomes: reversal by MPEP and CPCCOEt
| [3H]ACh-induced release | |
|---|---|
| 3,5-DHPG (10 μm) | 0.8 ± 8.9 |
| 3,5-DHPG (30 μm) | 29.6 ± 6.2a |
| 3,5-DHPG (100 μm) | 83.2 ± 17.3b |
| 3,5-DHPG (100 μm) + MPEP (1 μm) | 13.3 ± 5.3a |
| 3,5-DHPG (100 μm) + CPCCOEt (5 μm) | 16.5 ± 7.8a |
Antagonists were added 8 min before the agonist and maintained until the end of the superfusion period. Results are expressed as the percentage of increase over basal. Data are means ± SEM of four experiments run in triplicate.
↵ a p < 0.05 versus respective control
↵ b p < 0.01 versus respective control.