Table 1.

Concentrations of Aβ in the ISF, CSF, and tissue lysates in young and aged mice

Mean ± SEM concentration
3 months 12-15 months
Tissue-fluid 1−x40421−x4042
ISF-hippo (pg/ml) 727.7 ± 92.1 224.4 ± 23.3 93.6 ± 16.9 626.4 ± 93.9 345.9 ± 52.2 73.9 ± 38.9
ISF-striatum (pg/ml) 207.1 ± 31.8 142.1 ± 19.5
CSF (pg/ml) 11337 ± 699.7 5058 ± 496.0 2375 ± 336.8 8451 ± 915.3 4272 ± 898.7 961 ± 170.0
Hippo-carbonate (pg/mg protein) 255.0 ± 42.9 70.1 ± 14.5 80.3 ± 15.4 1147.6 ± 383.7 93.2 ± 29.7 692.5 ± 194.9
Hippo-guanidine (pg/mg protein) 1126 ± 135.9 105 ± 21.7 291 ± 30.4 87476 ± 29714 8159 ± 2775 66214 ± 22315
  • The concentration of ISF eAβ was assessed by microdialysis (n = 21). CSF was isolated from the cisterna magna and directly analyzed by ELISA (n = 6). Hippocampal tissues were processed by sequential extraction in carbonate buffer and then 5 m guanidine (n = 6). Tissue Aβ values are normalized to protein concentrations for each sample. Hippo, Hippocampus.