Table 1.

Linker between the subunits

Name Concatamer Sequences of linkers and adjacent subunits showing sites of linker integration (Added amino acids shown in bold) Linker length (C′ domain + linker + amino acids added in restriction sites = total)
α-12-β α4C-(AGS)12-Nβ2 WLAGMI-QEGT(AGS)12TG-TEERLV 8 + 36 + 6 = 50
β-6-α β2C-(AGS)6-Nα4 SAPSSK-EG(AGS)6-RAHAEE 23 + 18 + 2 = 43
α-6-β α4C-(AGS)6-Nβ2 WLAGMI-QEGT(AGS)6TG-TEERLV 8 + 18 + 6 = 32
β-α β2C-Nα4 SAPSSK-EG-AHAEER 23 + 0 + 2 = 25