Table 2.

mEPSC properties in GFP + cholinergic neurons in Cha-GAL4 cultures and in wild-type neurons examined in the absence of fluorescent illumination in Canton-S cultures

GFP+cholinergic neurons Cha-Gal4 mEPSCs (n = 11) Wild-type neurons Canton-S mEPSCs (n = 10)
Frequency (Hz) 0.66 ± 0.12 0.68 ± 0.19
Amplitude (pA) 27.5 ± 2.8 24.5 ± 2.7
Rise time (msec) 0.39 ± 0.07 0.3 ± 0.02
Decay τ (msec) 1.7 ± 0.21 1.5 ± 0.12
  • Recordings were obtained from GFP + cholinergic neurons in cultures prepared from Cha-GAL4 pupae and in neurons examined in the absence of fluorescent illumination in wild-type cultures prepared from Canton-S pupae. There were no significant differences in the mEPSC properties between these two groups or between either of these groups and the GFP + Kenyon cells in OK107-GAL4;UAS-GFP cultures (Table 1).