Table 1.

Extent of GFAP-cre-mediated deletion and loss of expression

Specimen	Type of analysis	Deletion or loss of expression^1-a
Astrocyte culture, 4 weeks, fl/fl; cre	IF	90% loss of expression (94% of GFAP⁺ cells)
Astrocyte culture, 6 weeks, fl/fl; cre	IF	90% loss of expression (94% of GFAP⁺ cells)
Astrocyte culture, 3 weeks, fl/fl; cre	WB	91% loss of expression
Astrocyte culture, 6 weeks, fl/fl; cre	WB	100% loss of expression
Cerebellum, adult, fl/−; cre	WB	100% loss of expression
Forebrain, adult, fl/−; cre	WB	90% loss of expression
Cerebellum, adult, fl/+; cre	SB	93% deletion
Forebrain, adult, fl/+; cre	SB	93% deletion
Astrocyte culture, 6 days, fl/+; cre	SB	89% deletion

IF, Immunofluorescence; WB, immunoblot; SB, Southern blot.
↵F1-a To determinehGFAP-cre-mediated loss of Cx43 expression, the expression of Cx43^fl/fl, hGFAP-cre, and Cx43^fl/−, hGFAP-cre specimens was always compared with the expression of Cx43^fl/fl and Cx43^fl/− specimens, respectively. The extent of deletion was assessed densitometrically as described previously (Betz et al., 1996) by the following equation: band intensity of Cx43^del fragment/(band intensities of Cx43^del fragment + Cx43^flfragment) × 100. All data are mean values from duplicate experiments (two animals).