Characterization of inspiratory bursts in wild-type and mutant embryonic mouse preparations
| n | Interval(s) | Duration(s) | Amplitude (mV) | Coefficient of variation of burst interval | |
|---|---|---|---|---|---|
| Wild-type en bloc | 5 | 3.2 ± 2.3 | 0.32 ± 0.09 | 46 ± 21 | 0.72 |
| Mutant en bloc | |||||
| Low frequency | 7 | 32 ± 41* | 0.33 ± 0.07 | 51 ± 34 | 1.3* |
| Medium frequency | 7 | 3.3 ± 2.4 | 0.28 ± 0.05 | 37 ± 26 | 0.73 |
| Combined average | 7 | 8.5 ± 17.3* | 0.28 ± 0.06 | 38 ± 27 | 2.0* |
| Wild-type slice | 5 | 3.5 ± 2.8 | 0.30 ± 0.08 | 33 ± 18 | 0.8 |
| Mutant slice | |||||
| Low frequency | 8 | 24 ± 37* | 0.29 ± 0.08 | 37 ± 21 | 1.5* |
| Medium frequency | 8 | 3.8 ± 2.8 | 0.26 ± 0.06 | 27 ± 20 | 0.74 |
| Combined average | 8 | 7.1 ± 15.6* | 0.27 ± 0.08 | 29 ± 20 | 2.2* |
The mean interburst interval, duration, and amplitude of inspiratory bursts were calculated from recordings of inspiratory motor discharge generated by brainstem–spinal cord (en bloc) and medullary slice preparations from E18.5 mice. The measurements for mutant mice were calculated separately for bouts of low- and medium-frequency bursting and put together as a combined average. Results are means ± SD; n is the number of preparations examined.
↵* p < 0.05 compared with wild type; Student's t test.