Table 2.

Pharmacological characterization of glutamate uptake into spinal cord synaptosomes prepared from transgenic G93A rats and age-matched controls

PDC (μm)DHK (μm)
End stage
 CON1.4  ± 0.174.2  ± 6.0
 G93A1.8  ± 0.175.3  ± 0.9
Onset
 CON1.6  ± 0.398.2  ± 5.3
 G93A2.4  ± 0.179.9  ± 8.1
Presymptomatic
 CON2.4  ± 0.587.9  ± 3.2
 G93A2.3  ± 0.389.8  ± 13.1
  • Pharmacological sensitivity of glutamate uptake in synaptosome fractions prepared from spinal cord of G93A and age-matched control (CON) rats was evaluated at various stages of disease progression. IC50 values were generated by nonlinear regression analysis of log concentration–response curves using the four-parameter logistic model. Data are mean ± SEM values from three independent experiments.