Table 1.

Evaluation of Na+-independentl-[3H]-glutamate uptake by systemx Embedded Image in ad-Nrf2-infected mixed immature cortical cultures: percentage increase and pharmacology of induced uptake

ad-GFPad-Nrf2ad-Nrf2DN
100%372  ± 82%*76.7  ± 7.1%*
Compoundad-GFP (%)ad-Nrf2 (%)
None100 372  ± 82
l-Cystine115.4  ± 13.286.5  ± 16.01-159
Quisqualic acid77.8  ± 10.871.8  ± 10.51-159
Homocysteic acid74.2  ± 22.080.0  ± 31.01-159
  • Data represent the mean ± SEM of five independent experiments performed in quadruplicate.

  • * p < 0.05, compared with ad-GFP control.

  • F1-159 p < 0.05, compared with ad-Nrf2 control with no compounds. Uptake assay performed on mixed neuron/glial immature cultures. The basal rate ofl-[3H]-glutamate uptake by ad-GFP-expressing cultures is 59.1 ± 5.7 fmol/mg protein per min.