Table 2.

Evaluation of GSH-bimane adduct staining in infected mixed cortical cultures

Cell typeAd-GFPAd-Nrf2Ad-Nrf2DN
Infected neuron21.1  ± 4.9 (60)40.6  ± 10 (60)*25.0 ± 5.2 (60)
Uninfected neuron19.0  ± 4.9 (60)34.1  ± 10 (60)*19.9 ± 4.5 (60)
Infected glia61.6  ± 11 (25)119  ± 17 (59)*35.1 ± 5.2 (40)*,2-159
  • Data represent the mean ± SEM, in arbitrary units of fluorescence intensity. Cell type was identified based on morphology (glial cells larger with web-like processes). The total number of cells evaluated for each group is listed in parentheses; with data collected from cells over three independent experiments performed in duplicate.

  • * p < 0.001, compared with ad-GFP of same cell type.

  • F2-159 p < 0.05, compared with ad-GFP of same cell type.