Table 1.

Estimation of the sensitivity of the combination of laser capture microdissection, T7 aRNA amplification, and cDNA microarray analysis using quantitative RT-PCR

Source IDMicroarray signalSEM of microarray signalcDNA copy numberSEM of cDNA copy number
M119311269.2137.42061.7156.9
AI029254337.530.9236.34.7
AA900184141.010.459.11.6
AA818955121.716.80.70.3
AA859035116.713.29.51.2
AI04450897.34.023.91.3
AA92579596.313.397.03.2
AI07191281.82.90.0
AI38513780.27.90.0
AA96471880.22.20.0
AA81929380.05.50.0
  • Eleven genes with a range of microarry expression levels from 1269.2 to 80 were quantified by quantitative RT-PCR on RNA from 30 laser captured cells from hippocampus CA1, captured in triplicate.